Human Adenylyl Cyclase Associated Protein 1 (CAP1) ELISA Kit | CAP1 elisa kit

Human Adenylyl Cyclase Associated Protein 1 (CAP1) ELISA Kit

Cat. Num. AAA2703217

• Gene Names: CAP1; CAP; CAP1-PEN
• Reactivity: Human
• Synonyms: Adenylyl Cyclase Associated Protein 1 (CAP1); Human Adenylyl Cyclase Associated Protein 1 (CAP1) ELISA Kit; CAP; CAP1-PEN; CAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylyl Cyclase Associated Protein 1 (CAP1).; No significant cross-reactivity or interference between Adenylyl Cyclase Associated Protein 1 (CAP1) and analogues was observed.
• Sequence Length: 475

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 1 (CAP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 1 (CAP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylyl Cyclase Associated Protein 1 (CAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylyl Cyclase Associated Protein 1 (CAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylyl Cyclase Associated Protein 1 (CAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylyl Cyclase Associated Protein 1 (CAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157649073
• NCBI GeneID: 10487
• NCBI Accession #: NP_001099000.1
• NCBI GenBank Nucleotide #: NM_001105530.1
• UniProt Accession #: Q01518; Q53HR7; Q5T0S1; Q5T0S2; Q6I9U6
• Molecular Weight: 51,830 Da
• NCBI Official Full Name: adenylyl cyclase-associated protein 1 isoform a
• NCBI Official Synonym Full Names: cyclase associated actin cytoskeleton regulatory protein 1
• NCBI Official Symbol: CAP1
• NCBI Official Synonym Symbols: CAP; CAP1-PEN
• NCBI Protein Information: adenylyl cyclase-associated protein 1
• UniProt Protein Name: Adenylyl cyclase-associated protein 1
• Protein Family: AP-1-like transcription factor
• UniProt Gene Name: CAP1
• UniProt Synonym Gene Names: CAP; CAP 1

NCBI Description

The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2016]

Uniprot Description

CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; extracellular exosome; extracellular region; focal adhesion; plasma membrane

Molecular Function: actin binding; adenylate cyclase binding

Biological Process: actin polymerization or depolymerization; adenylate cyclase activation; ameboidal cell migration; cell morphogenesis; establishment and/or maintenance of cell polarity; neutrophil degranulation; receptor-mediated endocytosis; signal transduction