Canine Adenylyl Cyclase Associated Protein 1 ELISA Kit | CAP1 elisa kit

Canine Adenylyl Cyclase Associated Protein 1 ELISA Kit

Cat. Num. AAA752467

• Gene Names: CAP1; CAP; CAP1-PEN
• Reactivity: Canine
• Synonyms: Adenylyl Cyclase Associated Protein 1; Canine Adenylyl Cyclase Associated Protein 1 ELISA Kit; CAP1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAP1 elisa kit

Intended Uses: This CAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CAP1. This ELISA kit for research use only!

Principle of the Assay: CAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAP1 antibody and an CAP1-HRP conjugate. The assay sample and buffer are incubated together with CAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAP1 concentration since CAP1 from samples and CAP1-HRP conjugate compete for the anti-CAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAP1 from the sample, fewer sites are left to bind CAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CAP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 157649073
• NCBI GeneID: 10487
• NCBI Accession #: NP_001099000.1
• NCBI GenBank Nucleotide #: NM_001105530.1
• Molecular Weight: 51,830 Da
• NCBI Official Full Name: adenylyl cyclase-associated protein 1
• NCBI Official Synonym Full Names: CAP, adenylate cyclase-associated protein 1 (yeast)
• NCBI Official Symbol: CAP1
• NCBI Official Synonym Symbols: CAP; CAP1-PEN
• NCBI Protein Information: adenylyl cyclase-associated protein 1; CAP 1
• UniProt Protein Name: Adenylyl cyclase-associated protein 1
• Protein Family: AP-1-like transcription factor
• UniProt Gene Name: CAP1
• UniProt Synonym Gene Names: CAP; CAP 1
• UniProt Entry Name: CAP1_HUMAN

NCBI Description

The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; focal adhesion; plasma membrane

Molecular Function: actin binding

Biological Process: platelet activation; axon guidance; receptor-mediated endocytosis; platelet degranulation; ameboidal cell migration; adenylate cyclase activation; establishment and/or maintenance of cell polarity; signal transduction; blood coagulation; actin cytoskeleton organization and biogenesis