Western Blot (WB)
(Western blot analysis of CAP1 expression in rat thymus extract (lane 1), rat spleen extract (lane 2) and SMMC7721 whole cell lysates (lane 3). CAP1 at 60KD was detected using rabbit anti-CAP1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit CAP1 Polyclonal Antibody | anti-CAP1 antibody
Anti-CAP1 Antibody
Gene Names
CAP1; CAP; CAP1-PEN
Reactivity
Human, Mouse, RatNo cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
CAP1; Polyclonal Antibody; Anti-CAP1 Antibody; CAP-1; CAP 1; CAP; CAP1 PEN; CAP1-PEN; Q01518; Adenylyl cyclase-associated protein 1; adenylate cyclase-associated protein 1 (yeast); anti-CAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
475
Applicable Applications for anti-CAP1 antibody
Western Blot (WB)
Application Notes
Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Immunogen
E Coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of CAP1 expression in rat thymus extract (lane 1), rat spleen extract (lane 2) and SMMC7721 whole cell lysates (lane 3). CAP1 at 60KD was detected using rabbit anti-CAP1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CAP1 expression in rat thymus extract (lane 1), rat spleen extract (lane 2) and SMMC7721 whole cell lysates (lane 3). CAP1 at 60KD was detected using rabbit anti-CAP1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-CAP1 antibody
Rabbit IgG polyclonal antibody for Adenylyl cyclase-associated protein 1 (CAP1) detection.
Background: Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.
Background: Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.
References
1. "Entrez Gene: CAP1 CAP, adenylate cyclase-associated protein 1 (yeast)".
2. Hubberstey A, Yu G, Loewith R, Lakusta C, Young D (Apr 1997). "Mammalian CAP interacts with CAP, CAP2, and actin". J Cell Biochem 61 (3): 459-66.
3. Matviw H, Yu G, Young D (Nov 1992). "Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins". Mol Cell Biol 12 (11): 5033-40.
2. Hubberstey A, Yu G, Loewith R, Lakusta C, Young D (Apr 1997). "Mammalian CAP interacts with CAP, CAP2, and actin". J Cell Biochem 61 (3): 459-66.
3. Matviw H, Yu G, Young D (Nov 1992). "Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins". Mol Cell Biol 12 (11): 5033-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,830 Da
NCBI Official Full Name
adenylyl cyclase-associated protein 1
NCBI Official Synonym Full Names
adenylate cyclase associated protein 1
NCBI Official Symbol
CAP1
NCBI Official Synonym Symbols
CAP; CAP1-PEN
NCBI Protein Information
adenylyl cyclase-associated protein 1
UniProt Protein Name
Adenylyl cyclase-associated protein 1
Protein Family
UniProt Gene Name
CAP1
UniProt Synonym Gene Names
CAP; CAP 1
UniProt Entry Name
CAP1_HUMAN
NCBI Description
The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Protein type: Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 1p34.2
Cellular Component: focal adhesion
Biological Process: adenylate cyclase activation; establishment and/or maintenance of cell polarity; signal transduction
Research Articles on CAP1
Similar Products
Product Notes
The CAP1 cap1 (Catalog #AAA178423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAP1 Antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat Tested Species:In-house tested species with positive results. Other applications have not been tested. Researchers should empirically determine the suitability of the CAP1 cap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
