Human Adenylate Cyclase 7, Brain (ADCY7) ELISA Kit | ADCY7 elisa kit

Human Adenylate Cyclase 7, Brain (ADCY7) ELISA Kit

Cat. Num. AAA163333

• Gene Names: ADCY7; AC7
• Reactivity: Human
• Synonyms: Adenylate Cyclase 7; Brain (ADCY7); Human Adenylate Cyclase 7; Brain (ADCY7) ELISA Kit; ADCY7 elisa kit

• Reactivity: Human
• Sequence Length: 1080

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 20ng/ml
• Sensitivity: 0.02ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY7 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Adenylate Cyclase 7, Brain (also known as ADCY7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.`

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ADCY7 antibody. ADCY7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ADCY7 Antibody is added and binds to ADCY7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADCY7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ADCY7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4557255
• NCBI GeneID: 113
• NCBI Accession #: NP_001105.1
• NCBI GenBank Nucleotide #: NM_001114.4
• UniProt Accession #: P51828; A0AVA6
• Molecular Weight: 120,308 Da
• NCBI Official Full Name: adenylate cyclase type 7 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 7
• NCBI Official Symbol: ADCY7
• NCBI Official Synonym Symbols: AC7
• NCBI Protein Information: adenylate cyclase type 7
• UniProt Protein Name: Adenylate cyclase type 7
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY7

NCBI Description

This gene encodes a membrane-bound adenylate cyclase that catalyses the formation of cyclic AMP from ATP and is inhibitable by calcium. The product of this gene is a member of the adenylyl cyclase class-4/guanylyl cyclase enzyme family that is characterized by the presence of twelve membrane-spanning domains in its sequences. Several transcript variants have been observed for this gene, but the full-length natures of only two have been determined so far. [provided by RefSeq, Oct 2013]

Uniprot Description

Catalyzes the formation of cAMP in response to activation of G protein-coupled receptors (Probable). Functions in signaling cascades activated namely by thrombin and sphingosine 1-phosphate and mediates regulation of cAMP synthesis through synergistic action of the stimulatory G alpha protein with GNA13 (PubMed:23229509, PubMed:18541530). Also, during inflammation, mediates zymosan-induced increase intracellular cAMP, leading to protein kinase A pathway activation in order to modulate innate immune responses through heterotrimeric G proteins G(12/13) (). Functions in signaling cascades activated namely by dopamine and C5 alpha chain and mediates regulation of cAMP synthesis through synergistic action of the stimulatory G protein with G beta:gamma complex (PubMed:23842570, PubMed:23229509). Functions, through cAMP response regulation, to keep inflammation under control during bacterial infection by sensing the presence of serum factors, such as the bioactive lysophospholipid (LPA) that regulate LPS-induced TNF-alpha production. However, it is also required for the optimal functions of B and T cells during adaptive immune responses by regulating cAMP synthesis in both B and T cells ().