Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit | ADAMTSL2 elisa kit

Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit

Cat. Num. AAA7213905

• Gene Names: ADAMTSL2; GPHYSD1
• Reactivity: Human
• Synonyms: ADAMTS like protein 2 (ADAMTSL2); Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit; ADAMTSL2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for ADAMTSL2 elisa kit

Intended Uses: This ADAMTSL2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTSL2. This ELISA kit for research use only!

Principle of the Assay: ADAMTSL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAMTSL2 antibody and an ADAMTSL2-HRP conjugate. The assay sample and buffer are incubated together with ADAMTSL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTSL2 concentration since ADAMTSL2 from samples and ADAMTSL2-HRP conjugate compete for the anti-ADAMTSL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTSL2 from the sample, fewer sites are left to bind ADAMTSL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTSL2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAMTSL2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 223718260
• NCBI GeneID: 9719
• NCBI Accession #: NP_001138792.1
• NCBI GenBank Nucleotide #: NM_001145320.1
• Molecular Weight: 104,621 Da
• NCBI Official Full Name: ADAMTS-like protein 2
• NCBI Official Synonym Full Names: ADAMTS-like 2
• NCBI Official Symbol: ADAMTSL2
• NCBI Official Synonym Symbols: GPHYSD1
• NCBI Protein Information: ADAMTS-like protein 2; ADAMTSL-2
• UniProt Protein Name: ADAMTS-like protein 2
• Protein Family: ADAMTS-like protein
• UniProt Gene Name: ADAMTSL2
• UniProt Synonym Gene Names: KIAA0605; ADAMTSL-2
• UniProt Entry Name: ATL2_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) and ADAMTS-like protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene lacks the protease domain, and is therefore of a member of the the ADAMTS-like protein subfamily. It is a secreted glycoprotein that binds the cell surface and extracellular matrix; it also interacts with latent transforming growth factor beta binding protein 1. Mutations in this gene have been associated with geleophysic dysplasia. [provided by RefSeq, Feb 2009]

Uniprot Description

ADAMTSL2: Defects in ADAMTSL2 are the cause of geleophysic dysplasia type 1 (GPHYSD1). An autosomal recessive disorder characterized by severe short stature, short hands and feet, joint limitations, and skin thickening. Radiologic features include delayed bone age, cone-shaped epiphyses, shortened long tubular bones, and ovoid vertebral bodies. Affected individuals have characteristic facial features including a 'happy' face with full cheeks, shortened nose, hypertelorism, long and flat philtrum, and thin upper lip. Other distinctive features include progressive cardiac valvular thickening often leading to an early death, toe walking, tracheal stenosis, respiratory insufficiency, and lysosomal-like storage vacuoles in various tissues.

Protein type: Secreted, signal peptide; Protease; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: proteinaceous extracellular matrix

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Geleophysic Dysplasia 1