Typical Testing Data/Standard Curve (for reference only)
Human Acrosin binding protein (ACRBP) ELISA Kit | ACRBP elisa kit
Human Acrosin binding protein (ACRBP) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ACRBP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACRBP antibody and an ACRBP-HRP conjugate. The assay sample and buffer are incubated together with ACRBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACRBP concentration since ACRBP from samples and ACRBP-HRP conjugate compete for the anti-ACRBP antibody binding site. Since the number of sites is limited, as more sites are occupied by ACRBP from the sample, fewer sites are left to bind ACRBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACRBP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse, guinea pig, and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues, this mRNA is expressed only in testis, whereas it is detected in a range of different tumor types such as bladder, breast, lung, liver, and colon. [provided by RefSeq, Jul 2008]
Uniprot Description
ACRBP: May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.
Protein type: Activator; Secreted, signal peptide; Secreted; Cancer Testis Antigen (CTA); Vesicle; Cell development/differentiation
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: acrosome; extracellular region; nucleus
Research Articles on ACRBP
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Product Notes
The Human ACRBP acrbp (Catalog #AAA7231544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231544 ELISA Kit recognizes Human ACRBP. It is sometimes possible for the material contained within the vial of "Acrosin binding protein (ACRBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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