Human ACRBP (Acrosin-binding protein) ELISA Kit | ACRBP elisa kit

Human ACRBP (Acrosin-binding protein) ELISA Kit

Cat. Num. AAA7612216

• Gene Names: ACRBP; CT23; SP32; OY-TES-1
• Reactivity: Human
• Synonyms: ACRBP (Acrosin-binding protein); Human ACRBP (Acrosin-binding protein) ELISA Kit; Acrosin-binding protein; 60 kDa form; Cancer/testis antigen 23; CT23; Cancer/testis antigen OY-TES-1; Proacrosin-binding protein sp32; mature form; 32 kDa form; ACRBP; ACRBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACRBP. No significant cross-reactivity or interference between ACRBP and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACRBP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 17999524
• NCBI GeneID: 84519
• NCBI Accession #: NP_115878.2
• NCBI GenBank Nucleotide #: NM_032489.2
• UniProt Accession #: Q8NEB7; Q9BY87
• Molecular Weight: 61,359 Da
• NCBI Official Full Name: acrosin-binding protein
• NCBI Official Synonym Full Names: acrosin binding protein
• NCBI Official Symbol: ACRBP
• NCBI Official Synonym Symbols: CT23; SP32; OY-TES-1
• NCBI Protein Information: acrosin-binding protein; cancer/testis antigen 23; cancer/testis antigen OY-TES-1; proacrosin binding protein sp32
• UniProt Protein Name: Acrosin-binding protein
• Protein Family: Acrosin-binding protein
• UniProt Gene Name: ACRBP
• UniProt Synonym Gene Names: CT23
• UniProt Entry Name: ACRBP_HUMAN

NCBI Description

The protein encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse, guinea pig, and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues, this mRNA is expressed only in testis, whereas it is detected in a range of different tumor types such as bladder, breast, lung, liver, and colon. [provided by RefSeq, Jul 2008]

Uniprot Description

ACRBP: May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.

Protein type: Cell development/differentiation; Cancer Testis Antigen (CTA); Secreted; Vesicle; Secreted, signal peptide; Activator

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: acrosome; extracellular region; nucleus