Human Caspase 1 (CASP1) ELISA Kit | CASP1 elisa kit

Human Caspase 1 (CASP1) ELISA Kit

Cat. Num. AAA165279

• Gene Names: CASP1; ICE; P45; IL1BC
• Reactivity: Human
• Synonyms: Caspase 1 (CASP1); Human Caspase 1 (CASP1) ELISA Kit; CASP1 elisa kit

• Reactivity: Human
• Sequence Length: 383

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml-15ng/ml
• Sensitivity: 0.018ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Carnosine Dipeptidase1 (also known as CNDP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CNDP1 antibody. CNDP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CNDP1 Antibody is added and binds to CNDP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CNDP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CNDP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 380254459
• NCBI GeneID: 834
• NCBI Accession #: NP_001244048.1
• NCBI GenBank Nucleotide #: NM_001257119.2
• UniProt Accession #: P29466; Q53EY6; Q6DMQ1; Q6GSS3; Q6PI75; Q9UCN3; B5MDZ1
• Molecular Weight: 42,888 Da
• NCBI Official Full Name: caspase-1 isoform beta
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).