Human ACP (Acid phosphatase) ELISA Kit | ACP elisa kit

Human ACP (Acid phosphatase) ELISA Kit

Cat. Num. AAA7606212

• Gene Names: NDUFAB1; ACP; ACP1; SDAP; FASN2A
• Reactivity: Human
• Synonyms: ACP (Acid phosphatase); Human ACP (Acid phosphatase) ELISA Kit; ACP elisa kit

• Reactivity: Human
• Sequence Length: 156

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ACP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ACP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ACP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21903370
• NCBI GeneID: 4706
• UniProt Accession #: O14561; Q9UNV1; B2R4M1
• Molecular Weight: 17,417 Da
• NCBI Official Full Name: Acyl carrier protein, mitochondrial
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit AB1
• NCBI Official Symbol: NDUFAB1
• NCBI Official Synonym Symbols: ACP; ACP1; SDAP; FASN2A
• NCBI Protein Information: acyl carrier protein, mitochondrial
• UniProt Protein Name: Acyl carrier protein, mitochondrial
• Protein Family: Sodium/proton-dependent alanine carrier protein
• UniProt Gene Name: NDUFAB1
• UniProt Synonym Gene Names: ACP

Uniprot Description

NDUFAB1: Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.

Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleoplasm

Molecular Function: acyl binding; acyl carrier activity; fatty acid binding; phosphopantetheine binding; protein binding

Biological Process: fatty acid beta-oxidation; glyoxylate metabolic process; lipid A biosynthetic process; mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; protein lipoylation