FGF6 active protein

FGF6 Protein, Human, Recombinant

Cat. Num. AAA8120842

• Gene Names: FGF6; HST2; HBGF-6
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF6; FGF6 Protein; Human; Recombinant; Human FGF6/FGF-6 Protein; HBGF-6 Protein; HST2 Protein; fibroblast growth factor 6; FGF6 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly41-Ile208

• Species: Human
• Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 1-6ng/mL.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human FGF6 (NP_066276.2)(Gly41-Ile208) was expressed with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 1-6 ng/mL.)

Related Product Information for FGF6 active protein

Background: FGF6, also known as FGF-6, belongs to the fibroblast growth factor (FGF) family. Members of this family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF6 plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis. It is also required for normal muscle regeneration. FGF6 gene displayed oncogenic transforming activity when transfected into mammalian cells.

References

Marics I, et al. (1989) Characterization of the HST-related FGF.6 gene, a new member of the fibroblast growth factor gene family. Oncogene. 4(3):335-40.Coulier F, et al. (1991) Putative structure of the FGF6 gene product and role of the signal peptide. Oncogene. 6(8):1437-44.Iida S, et al. (1992) Human hst-2 (FGF-6) oncogene: cDNA cloning and characterization. Oncogene. 7(2):303-9.

NCBI and Uniprot Product Information

• NCBI GI #: 15147343
• NCBI GeneID: 2251
• NCBI Accession #: NP_066276.2
• NCBI GenBank Nucleotide #: NM_020996.2
• UniProt Accession #: P10767; Q0VAE1
• Molecular Weight: 22,905 Da
• NCBI Official Full Name: fibroblast growth factor 6
• NCBI Official Synonym Full Names: fibroblast growth factor 6
• NCBI Official Symbol: FGF6
• NCBI Official Synonym Symbols: HST2; HBGF-6
• NCBI Protein Information: fibroblast growth factor 6
• UniProt Protein Name: Fibroblast growth factor 6
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF6
• UniProt Synonym Gene Names: HST2; HSTF2; FGF-6; HST-2; HSTF-2; HBGF-6

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF6: Plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: extracellular region

Molecular Function: 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell proliferation; cell-cell signaling; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphoinositide-mediated signaling; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; signal transduction