Mouse anti-Human Pbx1 Monoclonal Antibody | anti-PBX1 antibody

Pbx1

Cat. Num. AAA602443

• Gene Names: PBX1; MGC126627; DKFZp686B09108
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified using Protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
• Synonyms: Pbx1; Monoclonal Antibody; Anti -Pbx1; anti-PBX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8D105
• Specificity: Recognizes human PBX1.
• Purity/Purification: Affinity Purified; Purified using Protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
• Form/Format: Supplied as a liquid in PBS, 7.4, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-PBX1 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human PBX1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Related Product Information for anti-PBX1 antibody

Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.

Product Categories/Family for anti-PBX1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4505623
• NCBI GeneID: 5087
• NCBI Accession #: NP_002576.1
• NCBI GenBank Nucleotide #: NM_002585.3
• UniProt Accession #: P40424; Q5T488; Q68DD6
• Molecular Weight: 46,626 Da
• NCBI Official Full Name: pre-B-cell leukemia transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: pre-B-cell leukemia homeobox 1
• NCBI Official Symbol: PBX1
• NCBI Official Synonym Symbols: MGC126627; DKFZp686B09108
• NCBI Protein Information: pre-B-cell leukemia transcription factor 1; OTTHUMP00000032205; OTTHUMP00000032206; homeobox protein PRL; homeobox protein PBX1
• UniProt Protein Name: Pre-B-cell leukemia transcription factor 1
• Protein Family: Pre-B-cell leukemia transcription factor
• UniProt Gene Name: PBX1
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PBX1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

PBX1: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1. Expressed in all tissues except in cells of the B and T lineage. Belongs to the TALE/PBX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity

Biological Process: spleen development; transcription, DNA-dependent; thymus development; adrenal gland development; somatic stem cell maintenance; embryonic hemopoiesis; negative regulation of transcription factor activity; embryonic skeletal development; anterior/posterior pattern formation; negative regulation of neuron differentiation; ureteric bud branching; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; sex differentiation; steroid biosynthetic process; proximal/distal pattern formation; embryonic limb morphogenesis