Typical Testing Data/Standard Curve (for reference only)
Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13 (ADAMTS13) ELISA Kit | ADAMTS13 elisa kit
Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13 (ADAMTS13) ELISA Kit
Gene Names
ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
Reactivity
Human
Synonyms
A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13 (ADAMTS13); Human A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13 (ADAMTS13) ELISA Kit; ADAMTS13 elisa kit
Reactivity
Human
Sequence Length
1427
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml-200ng/ml
Sensitivity
0.36ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADAMTS13 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human A Disintegrin And Metalloprotease 17 (also known as ADAM17) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ADAM17 antibody. ADAM17 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ADAM17 Antibody is added and binds to ADAM17 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAM17antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ADAM17. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ADAM17 antibody. ADAM17 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ADAM17 Antibody is added and binds to ADAM17 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAM17antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ADAM17. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147,804 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 13
NCBI Official Symbol
ADAMTS13
NCBI Official Synonym Symbols
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Gene Name
ADAMTS13
UniProt Synonym Gene Names
C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
NCBI Description
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
Research Articles on ADAMTS13
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Product Notes
The Human ADAMTS13 adamts13 (Catalog #AAA166830) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166830 ELISA Kit recognizes Human ADAMTS13. It is sometimes possible for the material contained within the vial of "A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13 (ADAMTS13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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