TRAIL, soluble Active Protein | TNFSF10 active protein

TRAIL, soluble, Recombinant, Human (TRAIL, Apo-2L, TNFSF10, CD253)

Cat. Num. AAA637194

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
• Purity: Highly Purified; >98% (SDS-PAGE)
• Synonyms: TRAIL; soluble; Recombinant; Human (TRAIL; Apo-2L; TNFSF10; CD253); TNFSF10 active protein

• Host: Human, E Coli
• Specificity: Binds to human TRAIL receptors.
• Purity/Purification: Highly Purified; >98% (SDS-PAGE)
• Form/Format: In 20mM HEPES, pH 7.9, 120mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.
• Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

• Biological Activity: Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).
• Preparation and Storage: Short-term Storage: -20 degree C; Long-term Storage: -80 degree C; Once thawed it is recommended to prepare appropriate aliquots and to store them at -80 degree C.

Related Product Information for TNFSF10 active protein

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kD. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).

Product Categories/Family for TNFSF10 active protein

Molecular Biology; MB-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4507593
• NCBI GeneID: 8743
• NCBI Accession #: NP_003801.1
• NCBI GenBank Nucleotide #: NM_003810.3
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L
• UniProt Entry Name: TNF10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; apoptosis; positive regulation of apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus