Human Hypoxia Inducible Factor 2 Alpha (HIF2a) DIY ELISA Kit | HIF2a diy elisa kit
Human Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit DIY Materials
Gene Names
Epas1; HLF; Hif2a; HIF2 alpha; HIF-2 alpha
Reactivity
Human
Synonyms
Hypoxia Inducible Factor 2 Alpha (HIF2a); Human Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit DIY Materials; EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; HIF2a diy elisa kit
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.
Sequence Length
874
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for HIF2a diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
96,718 Da
NCBI Official Full Name
hypoxia inducible factor 2 alpha
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
Epas1
NCBI Official Synonym Symbols
HLF; Hif2a; HIF2 alpha; HIF-2 alpha
NCBI Protein Information
endothelial PAS domain-containing protein 1
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Gene Name
Epas1
UniProt Synonym Gene Names
Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha
NCBI Description
activates luciferase activity after transfection [RGD, Feb 2006]
Uniprot Description
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().
Research Articles on HIF2a
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Product Notes
The Human HIF2a epas1 (Catalog #AAA2089344) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089344 DIY ELISA Kit recognizes Human HIF2a. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the HIF2a epas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypoxia Inducible Factor 2 Alpha (HIF2a), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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