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Human GRB2 Related Adaptor Protein 2 (GRAP2) DIY ELISA Kit | GRAP2 diy elisa kit

Human GRB2 Related Adaptor Protein 2 (GRAP2) ELISA Kit DIY Materials

Gene Names
GRAP2; P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
Reactivity
Human
Synonyms
GRB2 Related Adaptor Protein 2 (GRAP2); Human GRB2 Related Adaptor Protein 2 (GRAP2) ELISA Kit DIY Materials; P38; GADS; GRAP-2; GRB2L; GRBLG; GRID; GRPL; GrbX; Grf40; Mona; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; GRAP2 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of GRB2 Related Adaptor Protein 2 (GRAP2). No significant cross-reactivity or interference between GRB2 Related Adaptor Protein 2 (GRAP2) and analogues was observed.
Sequence Length
330
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.061ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for GRAP2 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
24,382 Da
NCBI Official Full Name
Grb2-related adaptor protein 2
NCBI Official Synonym Full Names
GRB2-related adaptor protein 2
NCBI Official Symbol
GRAP2
NCBI Official Synonym Symbols
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
NCBI Protein Information
GRB2-related adapter protein 2
UniProt Protein Name
GRB2-related adapter protein 2
UniProt Gene Name
GRAP2
UniProt Synonym Gene Names
GADS; GRB2L; GRID; GRB2L; Grf-40

NCBI Description

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; endosome; nucleoplasm; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: cell-cell signaling; Ras protein signal transduction; T cell costimulation; T cell receptor signaling pathway

Research Articles on GRAP2

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Product Notes

The Human GRAP2 grap2 (Catalog #AAA2089397) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089397 DIY ELISA Kit recognizes Human GRAP2. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the GRAP2 grap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRB2 Related Adaptor Protein 2 (GRAP2), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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