Human Epithelial Neutrophil Activating Peptide 78 (ENA78) DIY ELISA Kit | ENA78 diy elisa kit

Human Epithelial Neutrophil Activating Peptide 78 (ENA78) ELISA Kit DIY Materials

Cat. Num. AAA2088886

• Gene Names: CXCL5; SCYB5; ENA-78
• Reactivity: Human
• Synonyms: Epithelial Neutrophil Activating Peptide 78 (ENA78); Human Epithelial Neutrophil Activating Peptide 78 (ENA78) ELISA Kit DIY Materials; CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys); Member 5; Neutrophil-activating peptide ENA-78; ENA78 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Epithelial Neutrophil Activating Peptide 78 (ENA78). No significant cross-reactivity or interference between Epithelial Neutrophil Activating Peptide 78 (ENA78) and analogues was observed.
• Sequence Length: 114

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for ENA78 diy elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4506849
• NCBI GeneID: 6374
• NCBI Accession #: NP_002985.1
• NCBI GenBank Nucleotide #: NM_002994.4
• UniProt Accession #: P42830; Q96QE1
• Molecular Weight: 11,972 Da
• NCBI Official Full Name: C-X-C motif chemokine 5
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 5
• NCBI Official Symbol: CXCL5
• NCBI Official Synonym Symbols: SCYB5; ENA-78
• NCBI Protein Information: C-X-C motif chemokine 5
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: CXCL5
• UniProt Synonym Gene Names: ENA78; SCYB5

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

Uniprot Description

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function: CXCR chemokine receptor binding

Biological Process: cell-cell signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; neutrophil mediated immunity; positive regulation of cell proliferation; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; signal transduction