Tyrosine Aminotransferase Active Protein | TAT active protein

Active Tyrosine Aminotransferase (TAT)

Cat. Num. AAA2122355

• Applications: Cell Culture, Activity Assays
• Purity: >95%
• Synonyms: Tyrosine Aminotransferase; Active Tyrosine Aminotransferase (TAT); Tyrosine Transaminase; L-tyrosine:2-oxoglutarate aminotransferase; TAT active protein

• Purity/Purification: >95%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence Positions: Cys221~Lys454
• Sequence Length: 454

• Applicable Applications for TAT active protein: Cell Culture, Activity Assays
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Cytosol, Memitochondrion
• Isoelectric Point: 5.5
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

(Figure. SDS-PAGE)

Western Blot (WB)

(Figure. Western Blot)

Biological Activity Testing

(Figure. The binding activity of TAT with HSPA8)

Product Categories/Family for TAT active protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4507369
• NCBI GeneID: 6898
• NCBI Accession #: NP_000344.1
• NCBI GenBank Nucleotide #: NP_000344.1
• UniProt Accession #: P17735
• Molecular Weight: Predicted Molecular Mass: 30.0kDa; Accurate Molecular Mass: 33kDa
• NCBI Official Full Name: tyrosine aminotransferase
• NCBI Official Synonym Full Names: tyrosine aminotransferase
• NCBI Official Symbol: TAT
• NCBI Protein Information: tyrosine aminotransferase
• UniProt Protein Name: Tyrosine aminotransferase
• Protein Family: Tyrosine aminotransferase
• UniProt Gene Name: TAT
• UniProt Synonym Gene Names: TAT

NCBI Description

This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]

Uniprot Description

TAT: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine. Defects in TAT are the cause of tyrosinemia type 2 (TYRO2); also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; EC 2.6.1.5; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: cytosol

Molecular Function: tyrosine transaminase activity

Biological Process: 2-oxoglutarate metabolic process; glutamate metabolic process; L-phenylalanine catabolic process; tyrosine catabolic process

Disease: Tyrosinemia, Type Ii