Human Endoglin (ENG) DIY ELISA Kit | ENG diy elisa kit

Human Endoglin (ENG) ELISA Kit DIY Materials

Cat. Num. AAA2088966

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Synonyms: Endoglin (ENG); Human Endoglin (ENG) ELISA Kit DIY Materials; CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1; ENG diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Endoglin (ENG). No significant cross-reactivity or interference between Endoglin (ENG) and analogues was observed.
• Sequence Length: 658

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for ENG diy elisa kit

Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 168693647
• NCBI GeneID: 2022
• NCBI Accession #: NP_001108225.1
• NCBI GenBank Nucleotide #: NM_001114753.2
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; focal adhesion; nucleoplasm; receptor complex

Molecular Function: activin binding; galactose binding; glycosaminoglycan binding; protein binding; punt binding; transforming growth factor beta binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: artery morphogenesis; atrial cardiac muscle morphogenesis; BMP signaling pathway; central nervous system vasculogenesis; heart looping; negative regulation of cell migration; negative regulation of protein amino acid autophosphorylation; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transforming growth factor beta receptor signaling pathway; smooth muscle development; sprouting angiogenesis; vasculogenesis; venous blood vessel morphogenesis

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber