Human Complement Component 1, Q Subcomponent A (C1qA) DIY ELISA Kit | C1qA diy elisa kit

Human Complement Component 1, Q Subcomponent A (C1qA) ELISA Kit DIY Materials

Cat. Num. AAA2089335

• Reactivity: Human
• Synonyms: Complement Component 1; Q Subcomponent A (C1qA); Human Complement Component 1; Q Subcomponent A (C1qA) ELISA Kit DIY Materials; q Subcomponent; Alpha Polypeptide; C1qA diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Component 1, Q Subcomponent A (C1qA). No significant cross-reactivity or interference between Complement Component 1, Q Subcomponent A (C1qA) and analogues was observed.
• Sequence Length: 245

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for C1qA diy elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 119615421
• NCBI GeneID: 712
• Molecular Weight: 26,017 Da
• NCBI Official Full Name: complement component 1, q subcomponent, A chain, isoform CRA_a
• NCBI Official Synonym Full Names: complement C1q A chain
• NCBI Official Symbol: C1QA
• NCBI Protein Information: complement C1q subcomponent subunit A
• UniProt Protein Name: Complement C1q subcomponent subunit A
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QA

NCBI Description

This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Uniprot Description

C1QA iso2: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2 )-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: complement component C1 complex; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell-cell signaling; complement activation; complement activation, classical pathway

Disease: C1q Deficiency