Rat Complement Component 1, Q Subcomponent A (C1qA) ELISA Kit | C1qA elisa kit

Rat Complement Component 1, Q Subcomponent A (C1qA) ELISA Kit

Cat. Num. AAA2023318

• Reactivity: Rat
• Synonyms: Complement Component 1; Q Subcomponent A (C1qA); Rat Complement Component 1; Q Subcomponent A (C1qA) ELISA Kit; q Subcomponent; Alpha Polypeptide; C1qA elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 1, Q Subcomponent A (C1qA).; No significant cross-reactivity or interference between Complement Component 1, Q Subcomponent A (C1qA) and analogues was observed.
• Sequence Length: 245

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.67ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C1qA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent A (C1qA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 1, Q Subcomponent A (C1qA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for C1qA elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 119615421
• NCBI GeneID: 712
• UniProt Accession #: P02745; Q5T963; B2R4X2
• Molecular Weight: 26,017 Da
• NCBI Official Full Name: complement component 1, q subcomponent, A chain, isoform CRA_a
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, A chain
• NCBI Official Symbol: C1QA
• NCBI Protein Information: complement C1q subcomponent subunit A
• UniProt Protein Name: Complement C1q subcomponent subunit A
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QA
• UniProt Entry Name: C1QA_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq, Jul 2008]

Uniprot Description

C1QA iso2: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2 )-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: collagen; extracellular region; complement component C1 complex

Molecular Function: protein binding

Biological Process: cell-cell signaling; innate immune response; complement activation, classical pathway; complement activation

Disease: C1q Deficiency