Human Chemokine C-C-Motif Ligand 16 (CCL16) DIY ELISA Kit | CCL16 diy elisa kit

Human Chemokine C-C-Motif Ligand 16 (CCL16) ELISA Kit DIY Materials

Cat. Num. AAA2089019

• Gene Names: CCL16; LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Ligand 16 (CCL16); Human Chemokine C-C-Motif Ligand 16 (CCL16) ELISA Kit DIY Materials; SCYA16; SCYL4; LEC; HCC4; LMC; LCC1; CKb12; Mtn1; CC-4; Monotactin-1; IL-10-inducible chemokine; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; CCL16 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 16 (CCL16). No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 16 (CCL16) and analogues was observed.
• Sequence Length: 120

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.070ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for CCL16 diy elisa kit

Cytokine; Tumor immunity; Infection immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 4759074
• NCBI GeneID: 6360
• NCBI Accession #: NP_004581.1
• NCBI GenBank Nucleotide #: NM_004590.3
• UniProt Accession #: O15467; Q4KKU0
• Molecular Weight: 13,600 Da
• NCBI Official Full Name: C-C motif chemokine 16
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 16
• NCBI Official Symbol: CCL16
• NCBI Official Synonym Symbols: LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16
• NCBI Protein Information: C-C motif chemokine 16
• UniProt Protein Name: C-C motif chemokine 16
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL16
• UniProt Synonym Gene Names: ILINCK; NCC4; SCYA16; HCC-4; LMC; MTN-1

NCBI Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL16: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemoattractant activity

Biological Process: cell communication; cell-cell signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of inflammatory response