Typical Testing Data/Standard Curve (for reference only)
General Immunoglobulin E ELISA Kit | IGHE elisa kit
Human Immunoglobulin E ELISA Kit
Gene Names
IGHE; IgE
Reactivity
General
Synonyms
Immunoglobulin E; Human Immunoglobulin E ELISA Kit; IGHE elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
428
Samples
Serum, plasma, hybridoma cell supernatants, ascites or other biological fluids
Preparation and Storage
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IGHE elisa kit
Intended Uses: This human immunoglobulin E antigen assay is intended for the quantitative determination of total human IgE antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids.
Principle of the Assay: Human IgE will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgE antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgE. Color development is proportional to the concentration of IgE in the samples.
Background: IgE is the least abundant immunoglobulin in serum and is predominately involved in the allergy response. IgE binds to allergens and triggers histamine release from mast cells and basophils. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections.
Principle of the Assay: Human IgE will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgE antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgE. Color development is proportional to the concentration of IgE in the samples.
Background: IgE is the least abundant immunoglobulin in serum and is predominately involved in the allergy response. IgE binds to allergens and triggers histamine release from mast cells and basophils. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections.
Product Categories/Family for IGHE elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
47,019 Da
NCBI Official Full Name
Ig epsilon chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant epsilon
NCBI Official Symbol
IGHE
NCBI Official Synonym Symbols
IgE
UniProt Protein Name
Ig epsilon chain C region
UniProt Gene Name
IGHE
UniProt Entry Name
IGHE_HUMAN
Uniprot Description
IGHE:
Protein type: Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: extracellular region
Molecular Function: antigen binding
Biological Process: innate immune response; immune response
Research Articles on IGHE
Similar Products
Product Notes
The General IGHE ighe (Catalog #AAA135520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135520 ELISA Kit recognizes General IGHE. It is sometimes possible for the material contained within the vial of "Immunoglobulin E, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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