Human Apolipoprotein L (APOL1) DIY ELISA Kit | APOL1 diy elisa kit
Human Apolipoprotein L (APOL1) ELISA Kit DIY Materials
Gene Names
APOLD1; VERGE
Reactivity
Human
Synonyms
Apolipoprotein L (APOL1); Human Apolipoprotein L (APOL1) ELISA Kit DIY Materials; Apo-L; APOL; APOL-I; APOL1 diy elisa kit
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein L (APOL1). No significant cross-reactivity or interference between Apolipoprotein L (APOL1) and analogues was observed.
Sequence Length
279
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
1.25-80ng/mL
Sensitivity
0.51ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for APOL1 diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,063 Da
NCBI Official Full Name
apolipoprotein L domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
apolipoprotein L domain containing 1
NCBI Official Symbol
APOLD1
NCBI Official Synonym Symbols
VERGE
NCBI Protein Information
apolipoprotein L domain-containing protein 1
UniProt Protein Name
Apolipoprotein L domain-containing protein 1
Protein Family
UniProt Gene Name
APOLD1
UniProt Synonym Gene Names
VERGE
NCBI Description
APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al., 2004 [PubMed 15102925]).[supplied by OMIM, Dec 2008]
Uniprot Description
APOLD1: May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood- brain permeability. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid-binding; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 12p13.1
Research Articles on APOL1
Similar Products
Product Notes
The Human APOL1 apold1 (Catalog #AAA2089349) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089349 DIY ELISA Kit recognizes Human APOL1. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the APOL1 apold1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein L (APOL1), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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