Human Apolipoprotein L (APOL1) ELISA Kit | APOL1 elisa kit

Human Apolipoprotein L (APOL1) ELISA Kit

Cat. Num. AAA2705212

• Gene Names: APOLD1; VERGE
• Reactivity: Human
• Synonyms: Apolipoprotein L (APOL1); Human Apolipoprotein L (APOL1) ELISA Kit; Apo-L; APOL; APOL-I; APOL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein L (APOL1).; No significant cross-reactivity or interference between Apolipoprotein L (APOL1) and analogues was observed.
• Sequence Length: 248

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.25-80ng/mL
• Sensitivity: < 0.51ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein L (APOL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein L (APOL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein L (APOL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein L (APOL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein L (APOL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein L (APOL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194353995
• NCBI GeneID: 81575
• NCBI Accession #: NP_001123887.1
• NCBI GenBank Nucleotide #: NM_001130415.1
• UniProt Accession #: Q96LR9; Q8IVR2; Q9H0I5
• Molecular Weight: 27,063 Da
• NCBI Official Full Name: apolipoprotein L domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: apolipoprotein L domain containing 1
• NCBI Official Symbol: APOLD1
• NCBI Official Synonym Symbols: VERGE
• NCBI Protein Information: apolipoprotein L domain-containing protein 1
• UniProt Protein Name: Apolipoprotein L domain-containing protein 1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOLD1
• UniProt Synonym Gene Names: VERGE

NCBI Description

APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al., 2004 [PubMed 15102925]).[supplied by OMIM, Dec 2008]

Uniprot Description

May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood-brain permeability.