Human Wingless Type MMTV Integration Site Family, Member 3 (WNT3) CLIA Kit | WNT3 clia kit

Human Wingless Type MMTV Integration Site Family, Member 3 (WNT3) CLIA Kit

Cat. Num. AAA2709226

• Gene Names: WNT3; INT4; TETAMS
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Wingless Type MMTV Integration Site Family; Member 3 (WNT3); Human Wingless Type MMTV Integration Site Family; Member 3 (WNT3) CLIA Kit; Member 3; INT4; Proto-oncogene Int-4 homolog; WNT3 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3 (WNT3).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3 (WNT3) and analogues was observed.
• Sequence Length: 355

• Applicable Applications for WNT3 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 41.2-30,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 15.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 3 (WNT3) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 16303266
• NCBI GeneID: 7473
• UniProt Accession #: P56703
• NCBI Official Full Name: WNT3
• NCBI Official Synonym Full Names: Wnt family member 3
• NCBI Official Symbol: WNT3
• NCBI Official Synonym Symbols: INT4; TETAMS
• NCBI Protein Information: proto-oncogene Wnt-3
• UniProt Protein Name: Proto-oncogene Wnt-3
• Protein Family: Proto-oncogene
• UniProt Gene Name: WNT3
• UniProt Synonym Gene Names: INT4
• UniProt Entry Name: WNT3_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; proteinaceous extracellular matrix; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; protein binding; frizzled binding; receptor agonist activity

Biological Process: embryonic forelimb morphogenesis; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; negative regulation of axon extension involved in axon guidance; embryonic hindlimb morphogenesis; Spemann organizer formation at the anterior end of the primitive streak; neuron differentiation; limb bud formation; mesoderm formation; gamete generation; anterior/posterior axis specification

Disease: Tetraamelia, Autosomal Recessive