Human SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit | SPD clia kit

Human SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit

Cat. Num. AAA2532756

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Reactivity: Human
• Synonyms: SPD (Pulmonary Surfactant Associated Protein D); Human SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit; SPD clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant SPD. No significant cross-reactivity or interference between SPD and analogues was observed.
• Sequence Length: 375

• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPD clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of SPD concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to SPD. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SPD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SPD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of SPD. You can calculate the concentration of SPD in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 317373510
• NCBI GeneID: 6441
• Molecular Weight: 37,728 Da
• NCBI Official Full Name: Pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_HUMAN

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22.2-q23.1

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; lysosome; endocytic vesicle; extracellular region

Molecular Function: protein binding; carbohydrate binding

Biological Process: receptor-mediated endocytosis; negative regulation of T cell proliferation; positive regulation of phagocytosis; defense response to bacterium; negative regulation of interleukin-2 biosynthetic process; innate immune response; respiratory gaseous exchange; alveolus development; macrophage chemotaxis; surfactant homeostasis; regulation of cytokine production