Rat Cytochrome C (CYCS) ELISA Kit | CYCS elisa kit

Rat Cytochrome C (CYCS) ELISA Kit

Cat. Num. AAA9712484

• Reactivity: Rat
• Synonyms: Cytochrome C (CYCS); Rat Cytochrome C (CYCS) ELISA Kit; CYCS; CYC; HCS; THC4; OTTHUMP00000202043; OTTHUMP00000202044; cytochrome c; CYCS elisa kit

• Reactivity: Rat
• Specificity: Rat Cytochrome c (CYCS) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYCS. No significant cross-reactivity or interference between Rat CYCS and analogues was observed.
• Sequence Length: 105

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Cytochrome c (CYCS) Standard Curve.)

Related Product Information for CYCS elisa kit

Description: This Rat Cytochrome c (CYCS) ELISA Kit employs a two-site sandwich ELISA to quantitate CYCS in samples. An antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Cytochrome c, or cyt c (horse heart: PDB 1HRC) is a small heme protein found loosely associated with the inner membrane of the mitochondrion. It is a soluble protein, unlike other cytochromes, and is an essential component of the electron transfer chain. It is capable of undergoing oxidation and reduction, but does not bind oxygen. It transfers electrons between Complexes III and IV. Cytochrome c is a highly conserved protein across the spectrum of species, found in plants, animals, and many unicellular organisms. This, along with its small size (molecular weight about 12,000 daltons), makes it useful in studies of evolutionary divergence. Its primary structure consists of a chain of 100 amino acids.

NCBI and Uniprot Product Information

• NCBI GI #: 119388072
• UniProt Accession #: P00008
• NCBI Official Full Name: Cytochrome c
• UniProt Protein Name: Cytochrome c
• Protein Family: Cytochrome
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC

Uniprot Description

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases ().