Western Blot (WB)
(Western blot analysis of Jurkat cells induced with 2mM of Camptothecin for 6 hours.)
Rabbit Active Caspase 7 Polyclonal Antibody
Active Caspase 7 antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Active Caspase 7 antibody was purified by affinity chromatography
Synonyms
Active Caspase 7; Polyclonal Antibody; Active Caspase 7 antibody; Polyclonal Active Caspase 7; anti-Active Caspase 7; CMH-1; ICE-LAP3; Active Caspase -7; CASP7; MCH3; EC 3.4.22.60; CASP-7; anti-Active Caspase 7 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Active Caspase 7 antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer containing 50% glycerol, 1% BSA, and 0.02% thimerosal
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-Active Caspase 7 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
IP: 10-20 ug/ml
IHC: 10-20 ug/ml
WB: 0.5-4 ug/ml
IHC: 10-20 ug/ml
WB: 0.5-4 ug/ml
Biological Significance
Caspase-7 has been identified as a major contributor to the execution of apoptosis. Caspase-7 is cleaved by many enzymes, including caspase-3, -6, -8, -9 and granzyme B. Active caspase-7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase-7 cleaves many of the same substrates as caspase-3, including poly polymerase. The active caspase-7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Immunogen
Active Caspase 7 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 195 of human caspase-7 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Jurkat cells induced with 2mM of Camptothecin for 6 hours.)
Western Blot (WB)
(Western blot analysis of Jurkat cells induced with 2mM of Camptothecin for 6 hours.)
Related Product Information for anti-Active Caspase 7 antibody
Rabbit polyclonal Active Caspase 7 antibody
Product Categories/Family for anti-Active Caspase 7 antibody
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Product Notes
The Active Caspase 7 (Catalog #AAA835766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Active Caspase 7 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Active Caspase 7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). IP: 10-20 ug/ml IHC: 10-20 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the Active Caspase 7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Active Caspase 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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