Typical Testing Data/Standard Curve (for reference only)
Human Immunoglobulin D (IgD) CLIA Kit | IgD clia kit
Human Immunoglobulin D (IgD)(CLIA) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Immunoglobulin D (IgD); Human Immunoglobulin D (IgD)(CLIA) CLIA Kit; CLIA Kit for Immunoglobulin D (IgD); IgD/Immunoglobulin D; IGHD; Constant Region Of Heavy Chain Of Immunoglobulin Delta; IgD clia kit
Reactivity
Human
Specificity
Reactive with Human Immunoglobulin D (IgD)
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin D (IgD). No significant cross-reactivity or interference between Immunoglobulin D (IgD) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin D (IgD). No significant cross-reactivity or interference between Immunoglobulin D (IgD) and analogues was observed.
Applicable Applications for IgD clia kit
Chemiluminescence Immunoassay (CLIA)
Application Notes
Chemiluminescent immunoassay for Antigen Detection.
Assay Type
Sandwich CLIA
Sample Types
serum, plasma and other biological fluids
Detection Method
Double-antibody Sandwich
Detection Range
31.2-2,000pg/mL
Sensitivity
13.4pg/mL
Assay Length
2.6 h
Sample Size
100ul (Please refer to the manual for the specific data.)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin D (IgD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin D (IgD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin D (IgD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly ADP Ribose Polymerase (PARP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Poly ADP Ribose Polymerase (PARP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for IgD clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
185,000
NCBI Official Full Name
Ig delta chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant delta
NCBI Official Symbol
IGHD
UniProt Protein Name
Ig delta chain C region
UniProt Gene Name
IGHD
UniProt Entry Name
IGHD_HUMAN
Uniprot Description
IGHD: IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: plasma membrane; integral to membrane
Molecular Function: antigen binding
Biological Process: immune response
Research Articles on IgD
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Product Notes
The Human IgD ighd (Catalog #AAA2709665) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709665 CLIA Kit recognizes Human IgD. AAA Biotech's Immunoglobulin D (IgD) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Chemiluminescent immunoassay for Antigen Detection. Researchers should empirically determine the suitability of the IgD ighd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Immunoglobulin D (IgD), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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