Typical Testing Data/Standard Curve (for reference only)
Human IFNalpha (Interferon Alpha) CLIA Kit | IFNalpha clia kit
Human IFNalpha (Interferon Alpha) CLIA Kit
Reactivity
Human
Synonyms
IFNalpha (Interferon Alpha); Human IFNalpha (Interferon Alpha) CLIA Kit; Human IFNalpha; IFNalpha clia kit
Reactivity
Human
Specificity
This kit recognizes Human IFNalpha in samples. No significant cross-reactivity or interference between Human IFNalpha and analogues was observed.
This CLIA kit applies to the in vitro quantitative determination of Human IFNalpha concentrations in serum, plasma and other biological fluids.
This CLIA kit applies to the in vitro quantitative determination of Human IFNalpha concentrations in serum, plasma and other biological fluids.
Assay Type
Sandwich CLIA
Detection Method
Chemiluminescence
Tested Sample Types
Serum, Plasma, Cell supernatant
Detection Range
7.81~500pg/mL
Sensitivity
4.69pg/mL
Precision
Both intra-CV and inter-CV are < 15%.
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFNalpha. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNalpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IFNalpha. You can calculate the concentration of Human IFNalpha in the samples by comparing the RLU value of the samples to the standard curve.
Preparation and Storage
An unopened kit can be stored at 2-8 degree C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the *following conditions (manual) once the kit is received.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
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Product Notes
The Human IFNalpha (Catalog #AAA2568126) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2568126 CLIA Kit recognizes Human IFNalpha. It is sometimes possible for the material contained within the vial of "IFNalpha (Interferon Alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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