Human GC (Glycocalicin) CLIA Kit | GC clia kit

Human GC (Glycocalicin) CLIA Kit

Cat. Num. AAA2530855

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• Reactivity: Human
• Synonyms: GC (Glycocalicin); Human GC (Glycocalicin) CLIA Kit; Carbohydrate-Rich Portion of Platelet Membrane Glycoprotein Ib Alpha Polypeptide; GC clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant GC. No significant cross-reactivity or interference between GC and analogues was observed.
• Sequence Length: 474

• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GC clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GC concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GC. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GC. You can calculate the concentration of GC in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 823672918
• NCBI GeneID: 2638
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: GC, partial
• NCBI Official Synonym Full Names: group-specific component (vitamin D binding protein)
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: lysosomal lumen; extracellular space; axon; perinuclear region of cytoplasm; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: lactation; response to nutrient levels; steroid metabolic process; female pregnancy; vitamin transport; response to estradiol stimulus; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1