Rabbit CLOCK Monoclonal Antibody | anti-CLOCK antibody

CLOCK Antibody

Cat. Num. AAA9436329

• Gene Names: CLOCK; KAT13D; bHLHe8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: CLOCK; Monoclonal Antibody; CLOCK Antibody; bHLHe8 antibody; Circadian locomoter output cycles kaput protein antibody; Circadian locomoter output cycles protein kaput antibody; Circadian Locomotor Output Cycles Kaput antibody; Circadium Locomotor Output Cycles Kaput antibody; Class E basic helix-loop-helix protein 8 antibody; CLOCK antibody; Clock circadian regulator antibody; Clock homolog antibody; Clock protein antibody; CLOCK_HUMAN antibody; hCLOCK antibody; KIAA0334 antibody; anti-CLOCK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: JA12-33
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-CLOCK antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of CLOCK on MCF-7 (1) and PC-12 (2) cell using anti-CLOCK antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CLOCK antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-CLOCK antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CLOCK antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLOCK antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining CLOCK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining CLOCK in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

Related Product Information for anti-CLOCK antibody

Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the Basic-helix-loop-helix-psp (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the clock gene have been shown to increase the length of the endogenous period and To contain a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation ability. It has been speculated that Clock may regulation circadian rhythmicity in combination with Other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye.

Product Categories/Family for anti-CLOCK antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 392841204
• NCBI GeneID: 9575
• NCBI Accession #: NP_001254772.1
• NCBI GenBank Nucleotide #: NM_001267843.1
• UniProt Accession #: O15516; O14516; Q9UIT8; A0AV01; A2I2N9
• Molecular Weight: 95,304 Da
• NCBI Official Full Name: circadian locomoter output cycles protein kaput
• NCBI Official Synonym Full Names: clock circadian regulator
• NCBI Official Symbol: CLOCK
• NCBI Official Synonym Symbols: KAT13D; bHLHe8
• NCBI Protein Information: circadian locomoter output cycles protein kaput; clock homolog; class E basic helix-loop-helix protein 8; circadian locomoter output cycles kaput protein
• UniProt Protein Name: Circadian locomoter output cycles protein kaput
• Protein Family: Clock-controlled protein
• UniProt Gene Name: CLOCK
• UniProt Synonym Gene Names: BHLHE8; KIAA0334; hCLOCK; bHLHe8
• UniProt Entry Name: CLOCK_HUMAN

NCBI Description

This gene product regulates circadian rhythm and metabolism. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and a DNA binding histone acetyltransferase. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: DNA-binding; Transcription factor; EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; chromosome; cytosol; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; response to redox state; signal transduction; activation of NF-kappaB transcription factor; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; photoperiodism; DNA damage checkpoint; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; circadian regulation of gene expression; histone acetylation; negative regulation of transcription, DNA-dependent; regulation of hair cycle; regulation of insulin secretion; positive regulation of inflammatory response