Human Galectin 9 (GAL9) CLIA Kit | GAL9 clia kit

Human Galectin 9 (GAL9) CLIA Kit

Cat. Num. AAA2708744

• Gene Names: LGALS9; HUAT; LGALS9A
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Galectin 9 (GAL9); Human Galectin 9 (GAL9) CLIA Kit; Galectin 9; LGALS9; Ecalectin; Lectin; Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21; GAL9 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Galectin 9 (GAL9).; No significant cross-reactivity or interference between Galectin 9 (GAL9) and analogues was observed.
• Sequence Length: 323

• Applicable Applications for GAL9 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.69-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.28pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Galectin 9 (GAL9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galectin 9 (GAL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galectin 9 (GAL9) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 102469694
• NCBI GeneID: 3965
• NCBI Accession #: NP_002299.2
• NCBI GenBank Nucleotide #: NP_002299.2
• UniProt Accession #: O00182
• NCBI Official Full Name: galectin-9 isoform short
• NCBI Official Synonym Full Names: galectin 9
• NCBI Official Symbol: LGALS9
• NCBI Official Synonym Symbols: HUAT; LGALS9A
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• Protein Family: Gallinacin
• UniProt Gene Name: LGALS9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm

Molecular Function: signal transducer activity; galactose binding; carbohydrate binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction