Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) CLIA Kit | CYFRA21-1 clia kit

Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) CLIA Kit

Cat. Num. AAA2708912

• Gene Names: KRT19; K19; CK19; K1CS
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Cytokeratin Fragment Antigen 21-1 (CYFRA21-1); Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) CLIA Kit; Cytokeratin Fragment Antigen 21-1; CYFRA21-1 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytokeratin Fragment Antigen 21-1 (CYFRA21-1).; No significant cross-reactivity or interference between Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) and analogues was observed.
• Sequence Length: 400

• Applicable Applications for CYFRA21-1 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.04-30ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.02ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 24234699
• NCBI GeneID: 3880
• NCBI Accession #: NP_002267.2
• NCBI GenBank Nucleotide #: NP_002267.2
• UniProt Accession #: P08727
• NCBI Official Full Name: keratin, type I cytoskeletal 19
• NCBI Official Synonym Full Names: keratin 19
• NCBI Official Symbol: KRT19
• NCBI Official Synonym Symbols: K19; CK19; K1CS
• NCBI Protein Information: keratin, type I cytoskeletal 19
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization