Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit | BMP-7 clia kit

Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit

Cat. Num. AAA2531758

• Gene Names: BMP7; OP-1
• Reactivity: Human
• Synonyms: BMP-7 (Bone Morphogenetic Protein 7); Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit; BMP7; OP1; Osteogenic Protein-1; BMP-7 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant BMP-7. No significant cross-reactivity or interference between BMP-7 and analogues was observed.
• Sequence Length: 431

• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-7 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of BMP-7 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to BMP-7. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of BMP-7. You can calculate the concentration of BMP-7 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502427
• NCBI GeneID: 655
• NCBI Accession #: NP_001710.1
• NCBI GenBank Nucleotide #: NM_001719.2
• Molecular Weight: 49,313 Da
• NCBI Official Full Name: bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: axon guidance; response to peptide hormone stimulus; negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; BMP signaling pathway; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; epithelial to mesenchymal transition; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; cell development; metanephros development; neurite morphogenesis; growth