Typical Testing Data/Standard Curve (for reference only)
Human BECN1 (Beclin 1) CLIA Kit | BECN1 clia kit
Human BECN1 (Beclin 1) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to BECN1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BECN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BECN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of BECN1. You can calculate the concentration of BECN1 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; mitochondrial membrane; autophagic vacuole; endosome membrane; phagocytic vesicle; trans-Golgi network; cytosol; endosome
Molecular Function: protein binding; ubiquitin protein ligase binding; phosphoinositide 3-kinase binding
Biological Process: viral reproduction; macroautophagy; late endosome to vacuole transport; cytokinesis; engulfment of apoptotic cell; mitotic metaphase plate congression; CVT pathway; regulation of cytokinesis; cellular response to nitrogen starvation; negative regulation of cell proliferation; cellular response to glucose starvation; lysosome organization and biogenesis; regulation of catalytic activity; receptor catabolic process; beta-amyloid metabolic process; autophagy; neuron development; cellular defense response; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation
Research Articles on BECN1
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Product Notes
The Human BECN1 becn1 (Catalog #AAA2531453) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531453 CLIA Kit recognizes Human BECN1. It is sometimes possible for the material contained within the vial of "BECN1 (Beclin 1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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