Human Albumin (ALB) CLIA Kit | ALB clia kit

Human Albumin (ALB) CLIA Kit

Cat. Num. AAA2707946

• Gene Names: ALB; HSA; PRO0883; PRO0903; PRO1341
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Albumin (ALB); Human Albumin (ALB) CLIA Kit; Albumin; Serum albumin; ALB clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).; No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
• Sequence Length: 609

• Applicable Applications for ALB clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.56-400ug/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.52ug/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Albumin (ALB). A competitive inhibition reaction is launched between biotin labeled Albumin (ALB) and unlabeled Albumin (ALB) (Standards or samples) with the pre-coated antibody specific to Albumin (ALB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Albumin (ALB) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Albumin (ALB) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4502027
• NCBI GeneID: 213
• NCBI Accession #: NP_000468.1
• NCBI GenBank Nucleotide #: NP_000468.1
• UniProt Accession #: P02768
• NCBI Official Full Name: serum albumin preproprotein
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: HSA; PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin
• UniProt Protein Name: Serum albumin
• Protein Family: Serum albumin
• UniProt Gene Name: ALB
• UniProt Entry Name: ALBU_HUMAN

NCBI Description

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor. [provided by RefSeq, Jul 2016]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Carrier; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic