Thrombopoietin Active Protein | TPO active protein

Human Thrombopoietin Protein, His Tag

Cat. Num. AAA9718678

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Thrombopoietin; Human Thrombopoietin Protein; His Tag; Recombinant Human Thrombopoietin Protein; His Tag (Thrombopoietin) expressed in Human Cells; MGDF; MKCSF; ML; MPLLG; THCYT1; THPO; TPO active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from mature form of human THPO (NP_000451.1) (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.
• Sequence Length: 353

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-60 ng/ml.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Thrombopoietin protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human Thrombopoietin protein, His Tag.)

Related Product Information for TPO active protein

Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.

NCBI and Uniprot Product Information

• NCBI GI #: 4507493
• NCBI GeneID: 7066
• NCBI Accession #: NP_000451.1
• NCBI GenBank Nucleotide #: NP_000451.1
• UniProt Accession #: P40225
• Molecular Weight: 37.6 kDa
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• Protein Family: Thrombopoietin

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]