TGFB1 active protein

Human TGFB1 Protein

Cat. Num. AAA9718693

• Gene Names: TGFB1; CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta
• Purity: >95% as determined by HPLC
• Synonyms: TGFB1; Human TGFB1 Protein; Recombinant Human TGFB1 Protein (Transforming growth factor beta-1) expressed in CHO Stable Cells; CED; DPD1; LAP; TGF-beta 1; TGFB; TGFbeta; Transforming growth factor beta-1; TGFB1 active protein

• Host: CHO Stable Cells
• Purity/Purification: >95% as determined by HPLC
• Form/Format: Lyophilized from sterile 100mM GLY, 10mM NaCl, pH 3.0
• Sequence: Amino acid sequence derived from active form of human/rhesus/canine TGFbeta1 (NP_000651.3) (Ala 279-Ser 390) was expressed and purified. Human, Rhesus and Canine TGFbeta1 sequences are identical.
• Sequence Length: 390

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human/rhesus/canine TGFbeta1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
• Activity: Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.04-0.2 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human TGFB1 protein in 0.1% TFA, 26% ACN, PH3.0 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human TGFB1 protein.)

Related Product Information for TGFB1 active protein

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-gamma, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

NCBI and Uniprot Product Information

• NCBI GI #: 63025222
• NCBI GeneID: 7040
• NCBI Accession #: NP_000651.3
• NCBI GenBank Nucleotide #: NP_000651.3
• UniProt Accession #: P01137
• Molecular Weight: 12.8 kDa
• NCBI Official Full Name: transforming growth factor beta-1 proprotein preproprotein
• NCBI Official Synonym Full Names: transforming growth factor beta 1
• NCBI Official Symbol: TGFB1
• NCBI Official Synonym Symbols: CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta
• NCBI Protein Information: transforming growth factor beta-1 proprotein; transforming growth factor beta-1
• Protein Family: Transforming growth factor

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]