TDGF1 active protein

TDGF1, human recombinant

Cat. Num. AAA845152

• Gene Names: TDGF1; CR; CRGF; CRIPTO
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: TDGF1; human recombinant; CRIPTO1; CRIPTO-1; CRGF; Teratocarcinoma-Derived Growth Factor 1; CR1.; TDGF1 active protein

• Host: Human cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. ; Appearance: Lyophilized protein
• Sequence: A DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1 - Thr 172) with a C-terminal polyhistidine tag was expressed.
• Sequence Length: 172

• Applicable Applications for TDGF1 active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Reconstitute in sterile PBS
• Biological Activity: Measured by its binding ability in a functional ELISA Immobilized human TDGF1 at 2 mug/ml (100 mul/well) can bind human ALK4 with a linear range of 0.0068 - 0.16 mug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -70 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for TDGF1 active protein

Background: Teratocarcinoma-derived growth factor 1, also known as epidermal growth factor-like cripto protein CR1, CRGF, and TDGF1, is a cell membrane which contains one EGF-like domain. In humans, TDGF1 is highly expressed in germ cell tumors and in colon and mammary carcinomas. TDGF1 is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. TDGF1 regulates the activation of several signaling pathways and controls cellular transformation in embryonic status. Patients with high TDGF1 expression were statistically susceptible to a recurrence of the disease, and showed poorer disease-free survival than those with low expression. TDGF1 is a predictive marker for metachronous metastasis in colorectal cancer (CRC) patients. It is preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. TDGF1 plays a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

Product Categories/Family for TDGF1 active protein

Cytokines; Growth Factors & Hormones; Cytokines; Growth Factors & Hormone (A-Z) Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Cytok

NCBI and Uniprot Product Information

• NCBI GI #: 292494883
• NCBI GeneID: 6997
• NCBI Accession #: NP_001167607.1
• NCBI GenBank Nucleotide #: NM_001174136.1
• UniProt Accession #: P13385; Q8TCC1
• Molecular Weight: 17.4 kDa
• NCBI Official Full Name: teratocarcinoma-derived growth factor 1 isoform 2
• NCBI Official Synonym Full Names: teratocarcinoma-derived growth factor 1
• NCBI Official Symbol: TDGF1
• NCBI Official Synonym Symbols: CR; CRGF; CRIPTO
• NCBI Protein Information: teratocarcinoma-derived growth factor 1
• UniProt Protein Name: Teratocarcinoma-derived growth factor 1
• Protein Family: Teratocarcinoma-derived growth factor
• UniProt Gene Name: TDGF1
• UniProt Synonym Gene Names: CRIPTO; CRGF
• UniProt Entry Name: TDGF1_HUMAN

NCBI Description

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.

Protein type: Membrane protein, GPI anchor; Cell adhesion; Cell cycle regulation; Motility/polarity/chemotaxis; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; in utero embryonic development; activation of MAPK activity; somatic stem cell maintenance; heart development; regulation of signal transduction; gastrulation; morphogenesis of a branching structure; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of fibroblast proliferation; cell migration during sprouting angiogenesis; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; cell differentiation; positive regulation of cell-matrix adhesion; positive regulation of cell migration; negative regulation of apoptosis