TDGF1 Antibody | anti-TDGF1 antibody

TDGF1 Antibody

Cat. Num. AAA8516127

• Gene Names: TDGF1; CR; CRGF; CRIPTO
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: TDGF1; TDGF1 Antibody; CR; CRGF; CRIPTO; Cripto-1; anti-TDGF1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: TDGF1 Antibody detects endogenous levels of total TDGF1
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 172

• Applicable Applications for anti-TDGF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human TDGF1
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of NCCIT cell lysate using TDGF1 antibody.)

Related Product Information for anti-TDGF1 antibody

Cripto, also known as teratocarcinoma derived growth factor 1 (TDGF-1), belongs to the EGF-CFC family of proteins. Members of this family are characterized by an N-terminal signal peptide, a conserved cysteine rich domain (CFC motif), and a short hydrophobic carboxy-terminal tail that contains GPI cleavage and attachment sites. The GPI moiety anchors Cripto and family members to the extracellular plasma membrane (1). An O-linked fucosylation site within the EGF-like motif is required for Cripto and related family members to perform their function as co-receptors for TGF-beta-related ligands such as Nodal and Vg1/GDF1 (2, 3). Soluble forms of Cripto can be produced-these contain intact EGF and CFC domains, and are thought to have paracrine activities, as opposed to the autocrine activity of Cripto functioning as a coreceptor (4). Understanding of this paracrine activity is not complete, but it is proposed that Cripto may act as co-ligand for Nodal (3).Cripto is an important modulator of embryogenesis and oncogenesis (4). It is highly expressed in early embryos, and in embryonic stem (ES) cells where it is involved in cardiomyocytic differentiation and acts as a negative regulator of neurogenesis (5-7). Transient activation of Cripto is essential for the capacity of stem cell self-renewal and pluripotency in ES cells, and in some adult derived stem cells (8). Signaling through Cripto can also stimulate other activities that promote tumorigenesis such as stimulation of proliferation, cell motility, invasion, angiogenesis and epithelial-mesenchymal transition (EMT) (9-11). Cripto is highly expressed in a broad range of tumors, where it acts as a potent oncogene.

NCBI and Uniprot Product Information

• NCBI GI #: 292494883
• NCBI GeneID: 6997
• NCBI Accession #: NP_001167607.1
• NCBI GenBank Nucleotide #: NM_001174136.1
• UniProt Accession #: P13385; Q8TCC1
• Molecular Weight: 21kDa
• NCBI Official Full Name: teratocarcinoma-derived growth factor 1 isoform 2
• NCBI Official Synonym Full Names: teratocarcinoma-derived growth factor 1
• NCBI Official Symbol: TDGF1
• NCBI Official Synonym Symbols: CR; CRGF; CRIPTO
• NCBI Protein Information: teratocarcinoma-derived growth factor 1
• UniProt Protein Name: Teratocarcinoma-derived growth factor 1
• Protein Family: Teratocarcinoma-derived growth factor
• UniProt Gene Name: TDGF1
• UniProt Synonym Gene Names: CRIPTO; CRGF

NCBI Description

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).