T Cell Immunoreceptor With Ig And ITIM Domains Active Protein | Tigit active protein

Recombinant Mouse T Cell Immunoreceptor With Ig And ITIM Domains Protein

Cat. Num. AAA2556756

• Gene Names: Tigit; Vstm3
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: T Cell Immunoreceptor With Ig And ITIM Domains; Recombinant Mouse T Cell Immunoreceptor With Ig And ITIM Domains Protein; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and transmembrane domain-containing protein 3; VSTM3; Tigit active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 241

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Gly26-Phe135
• Fusion Tag: C-6His
• Activity: Immobilized mouse TIGIT-His at 10 mug/ml (100 mul/well) can bind mouse PVR-Fch, The EC50 of mouse PVR-Fch is 0.31-0.73 mug/ml.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for Tigit active protein

Protein Construction: Recombinant Mouse T cell Immunoreceptor with Ig and ITIM domains is produced by our Mammalian expression system and the target gene encoding Gly26-Phe135 is expressed with a 6His tag at the C-terminus.

Background: T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called WUCAM, VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.

NCBI and Uniprot Product Information

• NCBI GI #: 226423924
• NCBI GeneID: 100043314
• NCBI Accession #: NP_001139797
• NCBI GenBank Nucleotide #: NM_001146325.1
• Molecular Weight: Molecular Mass: 13.3kDa; Actual Protein Molecular Mass: 18-22kDa
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: Tigit
• NCBI Official Synonym Symbols: Vstm3
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: Tigit
• UniProt Synonym Gene Names: Vstm3ImportedManual assertion inferred from database entriesiMGI:3642260

Uniprot Description

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.