Neutrophil Gelatinase-Associated Lpocalin Protein Active Protein | Lcn2 active protein

Recombinant Mouse Neutrophil Gelatinase-Associated Lipocalin Protein

Cat. Num. AAA2557420

• Gene Names: Lcn2; NRL; 24p3; Sip24; AW212229
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Neutrophil Gelatinase-Associated Lpocalin Protein; Recombinant Mouse Neutrophil Gelatinase-Associated Lipocalin Protein; Neutrophil gelatinase-associated lipocalin; NGAL; Lipocalin-2; SV-40-induced 24P3 protein; Siderocalin LCN2; p25; LCN2; Lcn2 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM MES, 150mM NaCl, 10% Glycerol, pH 5.5.
• Sequence: Gln21-Asn200
• Sequence Length: 200

• Species: Mouse
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-6His
• Activity: Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant mouse Lipocalin-2 can bind >1.0 muM of Fe(DHBA)3.
• Preparation and Storage: Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.

Related Product Information for Lcn2 active protein

Protein Construction: Recombinant Mouse Neutrophil gelatinase-associated lipocalin is produced by our mammalian expression system and the target gene encoding Gln21-Asn200 is expressed with a 6His tag at the C-terminus.

Background: Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), is a secretory protein of the lipocalin superfamily. Lipocalin-2 contains a signal peptide that enables it to be secreted and form complexes with matrix metalloproteinase-9 (MMP-9) through disulfide bonds. Similar to other lipocalin family members, Lipocalin-2 is involved in diverse cellular processes, including the transport of small hydrophobic molecules, protection of MMP-9 from proteolytic degradation, and cell signaling. Furthermore, Lipocalin-2 can tightly bind to bacterial siderophore through a cell surface receptor, possibly serving as a potent bacteriostatic agent by sequestering iron, regulating innate immunity and protecting kidney epithelial cells from ischemia-reperfusion injury. This protein is mainly expressed in neutrophils and in lower levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. Recent evidence also suggests its role as a biomarker for renal injury and inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 34328049
• NCBI GeneID: 16819
• NCBI Accession #: NP_032517.1
• NCBI GenBank Nucleotide #: NP_032517.1
• UniProt Accession #: P11672
• Molecular Weight: Molecular Mass: 21.9kDa; Actual Protein Molecular Mass: 23kDa
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: Lcn2
• NCBI Official Synonym Symbols: NRL; 24p3; Sip24; AW212229
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: Lcn2
• UniProt Synonym Gene Names: NGAL
• UniProt Entry Name: NGAL_MOUSE

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: iron ion binding; protease binding; protein binding; protein homodimerization activity

Biological Process: innate immune response; positive regulation of cell projection organization and biogenesis; response to drug; response to herbicide; response to virus; siderophore transport