IL-13 active protein

Mouse IL-13 Protein

Cat. Num. AAA9718720

• Gene Names: Il13; Il-13
• Purity: >90% as determined by SDS-PAGE
• Synonyms: IL-13; Mouse IL-13 Protein; Recombinant Mouse IL13 Protein (Interleukin-13) expressed in Human Cells; Il-13; Interleukin-13; IL13; IL-13 active protein

• Host: Human Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence: Amino acid sequence derived from mouse IL13 (P20109) (Pro22-Phe131) was expressed and purified.
• Sequence Length: 131

• Species: Mouse
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method; Protein Length
• Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 20-80 ng/mL. The ED50 for this effect is typically 2-10 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL13 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Mouse IL-13 protein.)

Related Product Information for IL-13 active protein

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains (IL-13 Ralpha1 and IL-13 Ralpha2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.

NCBI and Uniprot Product Information

• NCBI GI #: 6680403
• NCBI GeneID: 16163
• NCBI Accession #: NP_032381.1
• NCBI GenBank Nucleotide #: NP_032381.1
• UniProt Accession #: P20109
• Molecular Weight: 12.1 kDa
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: Il13
• NCBI Official Synonym Symbols: Il-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• UniProt Gene Name: Il13
• UniProt Synonym Gene Names: Il-13; IL-13
• UniProt Entry Name: IL13_MOUSE

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: interleukin-13 receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; positive regulation of protein secretion; positive regulation of B cell proliferation; positive regulation of ion transport; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; positive regulation of macrophage activation