IL-13 recombinant protein

Mouse IL-13 protein, His tag (Animal-Free)

Cat. Num. AAA9719009

• Gene Names: Il13; Il-13
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL-13; Mouse IL-13 protein; His tag (Animal-Free); ALRHMGC116789; BHR1interleukin-13; IL13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600; IL-13 recombinant protein

• Host: HEK 293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.
• Sequence: Met1-Phe131

• Endotoxin: < 1 EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from mouse IL13 protein (P20109, Met1-Phe131) was expressed with a polyhistidine tag at the C-terminus.
• Protein Length: The recombinant mouse IL-13 protein consists of 118 amino acids and predicts a molecular mass of 12.7 kD.The apparent molecular mass of the mouse IL13 is approximately 15-30kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized mouse IL-13 protein in the buffer which we provide not less than 100ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized mouse IL13 protein product should be stored desiccated below -18 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Mouse IL-13 protein, His tag)

Related Product Information for IL-13 recombinant protein

Description: Recombinant Human IL-13 protein expressed in HEK293 Cells.
Background: Interleukin(IL)-13, a cytokine produced by T helper 2 (Th2) cells, is a powerful inhibitor of macrophage functions, including surface expression of CD14 and production of IL-1 and tumor necrosis factor (TNF)-alpha. Interleukin (IL) -13 synergizes with IL2 in regulating interferon-gamma synthesis. It may be critical in regulating inflammatory and immune responses.Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity.IL-13 was first recognized for its effects on B cells and monocytes, where it upregulated class II expression, promoted IgE class switching and inhibited inflammatory cytokine production. It was also thought to be functionally redundant with IL-4.

Product Categories/Family for IL-13 recombinant protein

Cell Culture; Cytokines (Animal-Free)

NCBI and Uniprot Product Information

• NCBI GI #: 6680403
• NCBI GeneID: 16163
• NCBI Accession #: NP_032381.1
• NCBI GenBank Nucleotide #: NM_008355.3
• UniProt Accession #: P20109
• Molecular Weight: 14,108 Da
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: Il13
• NCBI Official Synonym Symbols: Il-13
• NCBI Protein Information: interleukin-13; T-cell activation protein P600
• UniProt Protein Name: Interleukin-13
• UniProt Gene Name: Il13
• UniProt Synonym Gene Names: Il-13; IL-13
• UniProt Entry Name: IL13_MOUSE

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: interleukin-13 receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; positive regulation of protein secretion; positive regulation of B cell proliferation; positive regulation of ion transport; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; positive regulation of macrophage activation