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Western Blot (WB) (Anti-LAF4 Antibody (C-term) at 1:2000 dilution + mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse LAF4 Polyclonal Antibody | anti-AFF3 antibody

LAF4 Antibody (C-term)

Gene Names
AFF3; LAF4; MLLT2-like
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Synonyms
LAF4; Polyclonal Antibody; LAF4 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); AF4/FMR2 family member 3; Lymphoid nuclear protein related to AF4; Protein LAF-4; AFF3; anti-AFF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This LAF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1197-1227 amino acids from the C-terminal region of human LAF4 .
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Sequence Positions
1197-1227
Applicable Applications for anti-AFF3 antibody
Western Blot (WB), ELISA
Application Notes
WB~~1:1000
Function
Putative transcription activator that may function in lymphoid development and oncogenesis. Binds, in vitro, to double- stranded DNA.
Cellular Location
Nucleus.
Tissue Location
Preferentially expressed in lymphoid tissues, highest levels being found in the thymus.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles .

Western Blot (WB)

(Anti-LAF4 Antibody (C-term) at 1:2000 dilution + mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LAF4 Antibody (C-term) at 1:2000 dilution + mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(The anti-LAF4 Pab is used in Western blot to detect LAF4 in placenta tissue lysate.)

Western Blot (WB) (The anti-LAF4 Pab is used in Western blot to detect LAF4 in placenta tissue lysate.)
Related Product Information for anti-AFF3 antibody
Lymphoid nuclear protein related to AF4 (LAF4) is related to MLLT2 which is also referred to as AF4. LAF4 is a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of LAF4 has defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis.
References
Hiwatari, M., et al., Oncogene 22(18):2851-2855 (2003). von Bergh, A.R., et al., Genes Chromosomes Cancer 35(1):92-96 (2002). Liao, X., et al., Mamm. Genome 7(6):467-468 (1996). Ma, C., et al., Blood 87(2):734-745 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133503
NCBI Official Full Name
AF4/FMR2 family member 3 isoform 2
NCBI Official Synonym Full Names
AF4/FMR2 family member 3
NCBI Official Symbol
AFF3
NCBI Official Synonym Symbols
LAF4; MLLT2-like
NCBI Protein Information
AF4/FMR2 family member 3
UniProt Protein Name
AF4/FMR2 family member 3
Protein Family
UniProt Gene Name
AFF3
UniProt Synonym Gene Names
LAF4; Protein LAF-4
UniProt Entry Name
AFF3_HUMAN

NCBI Description

This gene encodes a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of this protein initially defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis. In some ALL patients, this gene has been found fused to the gene for MLL. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AFF3: Putative transcription activator that may function in lymphoid development and oncogenesis. Binds, in vitro, to double- stranded DNA. Belongs to the AF4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: double-stranded DNA binding

Biological Process: embryonic hindlimb morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on AFF3

Similar Products

Product Notes

The AFF3 aff3 (Catalog #AAA9217019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1197-1227. The LAF4 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LAF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA. WB~~1:1000. Researchers should empirically determine the suitability of the AFF3 aff3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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