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Testing Data (Peptide-+)

anti-Human C/EBP-epsilon Antibody | anti-C/EBP-epsilon antibody

C/EBP-epsilon (Ab-74) Antibody

Gene Names
CEBPE; CRP1; C/EBP-epsilon
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
C/EBP-epsilon; C/EBP-epsilon (Ab-74) Antibody; C/EBP epsilon; CCAAT/enhancer binding protein epsilon; CEBPE; anti-C/EBP-epsilon antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
C/EBP-epsilon (Ab-74) antibody detects endogenous levels of total C/EBP-epsilon protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
281
Applicable Applications for anti-C/EBP-epsilon antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human C/EBP-epsilon around the phosphorylation site of threonine 74 (P-G-TP-P-A).
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using C/EBP-epsilon (Ab-74) antibody. Western blot analysis of extracts from HuvEc cells, treated with Insulin (0.01U/ml, 15mins), using C/EBP-epsilon (Ab-74) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using C/EBP-epsilon (Ab-74) antibody. Western blot analysis of extracts from HuvEc cells, treated with Insulin (0.01U/ml, 15mins), using C/EBP-epsilon (Ab-74) antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon (Ab-74) antibody.)

Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon (Ab-74) antibody.)
References
Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000. Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745-10751. Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125-133. Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,603 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein epsilon
NCBI Official Synonym Full Names
CCAAT enhancer binding protein epsilon
NCBI Official Symbol
CEBPE
NCBI Official Synonym Symbols
CRP1; C/EBP-epsilon
NCBI Protein Information
CCAAT/enhancer-binding protein epsilon
UniProt Protein Name
CCAAT/enhancer-binding protein epsilon
UniProt Gene Name
CEBPE
UniProt Synonym Gene Names
C/EBP epsilon

NCBI Description

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional activator (PubMed:26019275). C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).

Research Articles on C/EBP-epsilon

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Product Notes

The C/EBP-epsilon cebpe (Catalog #AAA856478) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The C/EBP-epsilon (Ab-74) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP-epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the C/EBP-epsilon cebpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP-epsilon, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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