Rabbit anti-Human ACR Polyclonal Antibody | anti-ACR antibody

ACR Antibody

Cat. Num. AAA9410535

• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: ACR; Polyclonal Antibody; ACR Antibody; Acrosin; anti-ACR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ACR protein.
• Form/Format: Supplied at 0.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 421

• Applicable Applications for anti-ACR antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000-1:2000; Western blotting: 1:200-1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Synthetic peptide corresponding to a region derived from 407-421 amino acids of human acrosin
• Target Name: ACR
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 12%SDS-PAGE Lysate: 50 ug Human fetal lung tissue lysate Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)

Related Product Information for anti-ACR antibody

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Product Categories/Family for anti-ACR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 148613878
• NCBI GeneID: 49
• NCBI Accession #: NP_001088
• NCBI GenBank Nucleotide #: NM_001097.2
• Molecular Weight: 45,847 Da
• NCBI Official Full Name: acrosin
• NCBI Official Synonym Full Names: acrosin
• NCBI Official Symbol: ACR
• NCBI Protein Information: acrosin
• UniProt Protein Name: Acrosin
• Protein Family: Acrosin
• UniProt Gene Name: ACR
• UniProt Synonym Gene Names: ACRS
• UniProt Entry Name: ACRO_HUMAN

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.10

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; acrosomal matrix; nucleus

Molecular Function: mannose binding; protein binding; copper ion binding; DNA binding; amidase activity; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding

Biological Process: binding of sperm to zona pellucida; response to steroid hormone stimulus; single fertilization; adenylate cyclase activation; acrosome reaction; penetration of zona pellucida; acrosome matrix dispersal; multicellular organism reproduction