MIA active protein
MIA protein
Applications
User optimized
Purity
> 98% pure
Synonyms
MIA; MIA protein; Cartilage-derived retinoic acid-sensitive protein protein; CD-RAP protein; Melanoma Inhibitory Activity protein; MIA active protein
Host
Human
Purity/Purification
> 98% pure
Form/Format
Lyophilized from 15mM Na3PO4, pH 7.2 with 75mM NaCl. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
Sequence
Region of MIA protein corresponding to amino acids MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ.
Applicable Applications for MIA active protein
User optimized
Protein Type
Recombinant
Biological Significance
MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Bioactivity
The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 ug/ml.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for MIA active protein
Purified recombinant Human MIA protein
Product Categories/Family for MIA active protein
Similar Products
Product Notes
The MIA (Catalog #AAA538101) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MIA can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the MIA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Region of MIA protein correspond ing to amino acids MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ. It is sometimes possible for the material contained within the vial of "MIA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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