FGF basic Active Protein | FGF active protein
FGF basic protein
Applications
User optimized
Purity
> 95% pure
Synonyms
FGF basic; FGF basic protein; FGF-2 protein; Prostatropin protein; Fibroblast Growth Factor-basic protein; HBGF-2 protein; FGF active protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Lyophilized from 5mM Tris, pH 7.6 with 150mM NaCl. Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1 - 1.0 mg/ml.
Sequence
Region of FGF basic protein corresponding to amino acids AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Applicable Applications for FGF active protein
User optimized
Protein Type
Recombinant
Biological Significance
FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF basic is a 17.2 kDa protein consisting of 154 amino acid residues.
Bioactivity
Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is approximately 0.5 ng/ml, corresponding to a specific activity of approximately 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is approximately 0.1 ng/ml, corresponding to a specific activity of approximately 1 x 10^7 units/mg.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for FGF active protein
Purified recombinant Human FGF basic protein
Product Categories/Family for FGF active protein
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Product Notes
The FGF (Catalog #AAA538111) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FGF basic can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the FGF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Region of FGF basic protein correspond ing to amino acids AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS. It is sometimes possible for the material contained within the vial of "FGF basic, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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